According to the WHO, influenza is one of the most pressing problems in world health. Every year, about 500 thousand people die due not only to the infection itself, but also from the complications it causes [1]. Because of their high variability, influenza viruses rapidly develop resistance to many traditional anti-influenza therapies [2]. The development of drugs based on the mechanism of gene silencing using siRNA is a promising direction. Objective: To assess the decrease in viral reproduction in cells with reduced expression of the genes of the nuclear-pore complex Nup98 and Nup205. Materials and methods. Viruses. We used the influenza A / WSN / 1933 virus strain (St. Jude’s Children’s Research Hospital, USA). Cell lines. A549 cell culture - human lung adenocarcinoma (ATCC® CCL-185, USA), MDCK cell culture – dogs ren (Institut Pasteur, France). siRNA. Sequences (Nup98.1 (miRNA1), Nup98.2 (miRNA2), Nup205) to mRNA of target genes (Syntol, RF). Nonspecific siRNA L2 was also synthesized to assess the functionality of working miRNAs. Transfection agent.
